AN INVESTIGATION INTO THE CHARACTERIZATION OF BIOACTIVE COMPOUNDS OF CITRUS SINENSIS (ORANGE) PEEL USING MASS SPECTROMETER

Student: Chidera Miracle Okpara
Supervisor: Mrs Ikeyi A P
HOD: Dr Arinzeagu Christian Chukwuma
Department of SCIENCE AND LABORATORY TECHNOLOGY
Science and Technology
Institute of Management and Technology, Enugu, Enugu State

Abstract

The present study analyzed the bioactive composition of Citrus sinensis leaves using Gas Chromatography-Mass Spectrometry (GC-MS), revealing a diverse range of bioactive compounds with significant pharmaceutical potential. Predominant compounds identified included Pinene (12.21%), Lunamarin (12.67%), and Caryophyllene (2.43%), which are known for their anti inflammatory, antimicrobial, and anticancer properties. Other significant compounds such as Carene (8.22%) and Oxalic acid derivatives (2.02%) further highlighted the bioactive richness of Citrus sinensis leaves. Comparative analysis with previous studies demonstrated variability in bioactive composition, with differences attributed to variations in extraction methods, geographical factors, and plant maturity. This study underscores the therapeutic potential of Citrus sinensis leaves while highlighting the need for standardized analytical methods and the use of multiple analytical techniques such as LC-MS and NMR to achieve more consistent and comprehensive profiling of bioactive compounds. Future pharmacological studies are recommended to explore the specific therapeutic applications of the identified compounds.

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