THE USE OF SUCROSE AS SUBSTRATE FOR GLYCOPROPTEIN BIOSURFACTANT PRODUCTION BY ENTROBACTER
Abstract
This study investigated the use of sucrose as a carbon substrate for glycoprotein biosurfactant production by Enterobacter species. Biosurfactants are microbial surface-active compounds with broad industrial applications due to their biodegradability, low toxicity, and stability. Sucrose was examined as an inexpensive carbon source to enhance microbial growth and biosurfactant synthesis.
Enterobacter isolates were cultured on sucrose-based media, and growth parameters measured included lawn formation (L.O), colony counts, and viable cell enumeration (CFU/mL). Two treatments—TB and UMC—were evaluated at a dilution factor of 10⁵. TB recorded a lawn formation of 340 and CFU value of 3.4 × 10⁶ CFU/mL, while UMC showed 128 and 1.2 × 10⁶ CFU/mL, respectively. The results indicate that sucrose supported bacterial proliferation in both treatments, with TB demonstrating significantly higher biomass and potential biosurfactant yield.
The findings confirm sucrose as an effective, low-cost substrate for promoting growth and biosurfactant production by Enterobacter species. This suggests its applicability in large-scale industrial biosurfactant production. Recommendations for optimization and future studies are also provided.
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