MOLECULAR DETECTION OF VIRUS INFECTING YAM (DIOSCOREA SPP.) IN AKPABUYO, CROSS RIVER STATE
Abstract
Yam (Dioscorea spp.) is an important food crop cultivated for its edible tubers in
Cross River State, Nigeria. A visit to some yam farms during the 2024 farming
season revealed evidence of virus infection resulting in poor yield. The study was
aimed at detecting virus infecting yam in Akpabuyo Cross River State. The infected
leaf samples were tested against YMV and YMMV specific primers using RT-PCR.
The result showed that the sample tested positive to the YMV Primer confirming
hat the virus under study is yam mosaic virus (YMV). The sequence obtained when
compared with other virus sequences available in the NCBI GenBank using
BLASTn revealed 92 % sequence identity with Yam mosaic Virus (YMV)
confirming that the virus under study is YMV. This is the first report of detection
of virus infecting yam in Cross River State.
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