PRODUCTION AND ANTI-MICROBIAL ACTIVITY OF HERBAL SHAMPOO FROM COW MILK AND GARLIC

ABSTRACT This study aimed to formulate and evaluate an herbal antimicrobial shampoo using cow milk and garlic (Allium sativum) as primary active ingredients. The shampoo was prepared through the decoction extraction method, combining cow milk, garlic extract, Amla, Reetha, sandalwood oil, and star anise oil. Physicochemical parameters such as appearance, pH, foam stability, conditioning action, and skin irritation were assessed, while antimicrobial activity was tested against Staphylococcus aureus and Malassezia furfur. The formulated shampoo exhibited a brownish, opaque appearance with a near-neutral pH (7.35), stable foam formation, and no signs of skin irritation, indicating its safety for topical use. Antimicrobial analysis showed dose-dependent inhibition of both organisms, with M. furfur being more sensitive. The Minimum Inhibitory Concentration (MIC) values were 2.5 mg/ml for S. aureus and 1.8 mg/ml for M. furfur, confirming significant antimicrobial potential. The combined effects of allicin in garlic and bioactive compounds in cow milk contributed to the observed antibacterial and antifungal activity. Overall, the formulated shampoo demonstrated promising cleansing, conditioning, and antimicrobial properties, suggesting that it can serve as a safe, eco-friendly, and effective alternative to conventional synthetic shampoos.