ISOLATION AND IDENTIFICATION OF GRAM POSITIVE BACTERIA IN POULTRY FEED IN IBADAN SOUTH WEST AND IDO LOCAL GOVERNMENT AREAS, OYO STATE.
Abstract
Poultry farming is a significant agricultural activity in Nigeria, particularly in Oyo State, providing essential protein sources and supporting the livelihoods of many households. This study aims to isolate and identify gram-positive bacteria in poultry feed samples from two Local Government Areas in Ibadan: South West and Ido Local Government Areas, Ibadan, Oyo State. The objectives of this study are to: identify the types of gram-positive bacteria present in poultry feed; determine the prevalence of these bacteria in different sources of feed (farms, feed mills, and vendors); and assess the potential health risks associated with these contaminants.
A total of 60 poultry feed samples were collected using a snowball sampling technique from various sources, including farms, vendors, and feed mills. The samples were subjected to laboratory analysis using standard microbiological methods, including serial dilution and pre-pour plate methods. The identified bacteria were then characterized based on their morphological and biochemical properties.
The results revealed a high prevalence of gram-positive bacteria in the poultry feed samples, with significant variations in bacterial load among the different sources. The most common isolates included Staphylococcus aureus, Bacillus spp., and Enterococcus spp. The highest bacterial counts were observed in samples from vendors, followed by feed mills and farms.
This study revealed a high level of contamination of poultry feed collected from Ibadan South West and Ido Local Government Areas with Gram-positive bacteria. The presence of these microbes in the feed poses a health hazard for poultry birds, poultry handlers, and consumers.
This study recommends that regular surveillance of bacterial contamination levels in poultry feed should be conducted to promptly identify and address potential issues, ensuring feed safety and protecting public health.
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