ISOLATION AND IDENTIFICATION OF BACTERIA AND FUNGI IN EFFLUENTS COLLECTED FROM ABATTOIRS IN BENIN CITY
Abstract
This study investigated the microbial diversity and load in effluents collected from three
abattoirs in Ikpoba Hill, Benin City, using standard culture-based methods. A total of three
composite samples, one from each abattoir, were analyzed on Nutrient Agar, MacConkey Agar,
and Potato Dextrose Agar for bacterial and fungal isolation. The microbial load ranged from
33.33 × 10⁵ CFU/mL in Abattoir 1 to 54.33 × 10⁵ CFU/mL in Abattoir 3, with Abattoir 3
recording the highest load and Abattoir 1 the lowest. Abattoir 2 showed an intermediate count
of 41.67 × 10⁵ CFU/mL. Bacterial isolates included Shigella spp., Pseudomonas spp.,
Klebsiella spp., Proteus spp., Corynebacterium spp., Micrococcus spp., Staphylococcus
aureus, Serratia spp., and Salmonella spp. Fungal isolates comprised Fusarium spp.,
Penicillium spp., Aspergillus spp., Cladosporium spp., and Mucor spp. The results indicate that
untreated abattoir wastewater harbors a wide range of pathogenic and opportunistic
microorganisms, posing potential public health and environmental risks. Strengthened waste
management practices and effective treatment systems are recommended to mitigate microbial
contamination before effluent discharge.
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