MICROBIAL AND PHYSICOCHEMICAL ASSESSMENT OF SOIL SAMPLES COLLECTED FROM DIFFERENT PALM OIL PRODUCTION SITE IN UMUAGWO VILLAGE

Student: Favour Nmesoma Okeorlu
Supervisor: Dr Udochukwu Ugueri
HOD: Dr V.n Unegbu
Department of Microbiology
Sciences
UNIVERSITY OF AGRICULTURE AND ENVIRONMENTAL SCIENCES, UMUAGWO, IMO STATE

Abstract

This study investigated the visual, physico-chemical, and microbial characteristics of soil samples collected from three palm oil mill sites (Sites A, B, and C) to assess the impact of oil mill activities on soil quality and microbial ecology. A total of 18 soil samples were examined for visual properties (color, odour, moisture, and debris content), physicochemical parameters (pH, temperature, moisture content, and water holding capacity), and microbial load (total heterotrophic bacterial and fungal counts). In addition, isolated microbial species were identified based on their cultural, morphological, and emical characteristics. The results showed that soil samples exhibited varying degrees of dark coloration and odour, indicating high levels of organic matter contamination. Soil pH ranged from strongly acidic (3.50) to near neutral (7.00), while moisture content and water holding capacity varied significantly across sites. Microbiological analysis revealed bacterial counts ranging from 1.6 × 10⁴ to 7.3 × 10⁴ cfu/g and fungal counts between 1.8 × 10¹ and 1.3 × 10³ cfu/g. Common bacterial isolates included Pseudomonas spp., Bacillus spp., Klebsiella spp., E. coli, and Staphylococcus aureus, while fungal isolates included Aspergillus spp., Rhizopus spp., Penicillium spp., and Trichoderma spp. The study concludes that palm oil mill activities significantly alter soil characteristics, introducing organic pollutants and fostering the proliferation of microbial communities. The presence of hydrocarbon-degrading microbes suggests potential for natural bioremediation. The study recommends improved waste management practices, soil pH remediation, and further research into the biotechnological applications of indigenous microorganisms

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