OPTIMIZATION OF FERMENTATION CONDITIONS FOR EXOGLUCANASE PRODUCTION BY TRICHODERMA ASPERELLUM ISOLATED FROM DEGRADED (RAUVOLFIA VOMITORIA).
Abstract
ABSTRACT
Cellulases have numerous applications in several industries, including biofuel production, food and feed industry, brewing, pulp and paper, textile, laundry, and agriculture. Cellulose-degrading bacteria are widely spread in nature, being isolated from quite different environments. Cellulose degradation is the result of a synergic process between an endoglucanase, an exoglucanase and a β-glucosidase. Bacterial endoglucanases degrade β-1,4-glucan linkages of cellulose amorphous zones, meanwhile exoglucanases cleave the remaining oligosaccharide chains, originating cellobiose, which is hydrolyzed by β-glucosidases. Bacterial cellulases (EC 3.2.1.4) are comprised in fourteen Glycose Hydrolase families. Several advantages, such as higher growth rates and genetic versatility, emphasize the suitability and advantages of bacterial cellulases over other sources for this group of enzymes. This project revealed optimization conditions for exoglucanase production. The optimal conditions for exoglucanase production by T. asperellum were found to be:
•120 hours of cultivation
• pH 6.0–7.0
• 4% inoculum size
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