ISOLATION AND IDENTIFICATION OF MULTI-DRUG E COLI FROM CLINICAL SAMPLES AMONG PATIENT ATTENDING STATES SPECIALIST HOSPITAL MAIDUGURI

Abuse and improper prescribing policy of antibiotics causes remarkable increase of antibiotic resistance pattern among the E. coli isolates from Urinary Tract Infection (UTI). This study was conducted to isolate and identify multi drug resistant MDR Escherichia coli from urine and stool sample of patients by convention and traditional techniques. fourty (40) urine samples were collected from patients admitted to nearby hospitals. Identification of isolated strains were determined by biochemical tests like Gram staining, indole, catalase, and urease. Sensitivity pattern of isolates were determined against some traditional and conventional antibiotics. From this study, it was revealed that 56.75% isolates were gram negative. Indole positivity was observed for 95.23% of Gram negative isolates. 100% of indole positive isolates were positive for catalse, negative carbohydrate fermentation on broth medium and carbohydrate utilization on Triple sugar iron agar. Negativity was seen for oxidase, urease, citrate, and gelatinase test. It was also observed that 100% of indole positive isolates gave positivity on differential media such as Mac Conkey agar and EMB agar. Antibiotic sensitivity indicates 85% of identifyed strains were multi drug resistant. The findings of the study suggested that 15 MDR E. coli were identified and all of them were multi drug resistant strains.