UTILIZATION OF NEGLECTED PLANTAIN (MUSA PARADISIACA) PEEL AND STEM FOR PULLULANASE AND MANNANASE PRODUCTION IN SUBMERGED FERMENTATION.
Abstract
Enzymes are organic catalysts with numerous industrial applications and are required in large quantities. Due to increasing demand, there is a need to explore alternative low-cost substrates for enzyme production. Agricultural wastes such as lignocellulosic biomasses offer sustainable solutions for enzyme biotechnology.
This study investigated the utilization of neglected plantain (Musa paradisiaca) peel and stem as substrates for the production of pullulanase and mannanase enzymes using submerged fermentation. The plantain biomass was pretreated to enhance accessibility of fermentable sugars, and bacterial isolates capable of producing the enzymes were screened and optimized for production under varying substrate concentrations, pH levels, temperatures, and incubation periods.
Results showed significant enzyme yields with maximum pullulanase and mannanase activities under optimized fermentation conditions. The findings demonstrated that plantain peel and stem can serve as viable and low-cost substrates for industrial enzyme production.
This research highlights the potential of converting agricultural waste into valuable biotechnological resources while reducing environmental pollution associated with agro-waste disposal.
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