31751813673
Abstract
Keratinase is an extra-cellular protease, having efficient ability to degrade keratin which are found in feathers, wool, nail and hair. It has various areas of application including leather, textile, pharmaceutical, detergent and animal feed industries. Previous studies have centred on isolation of keratinase producing fungi and bacterial, leaving a gap in the aspect of screening and optimization. This study isolated, screened, and optimized growth conditions of keratinase producing bacteria from poultry waste,with a view to identifying keratinase - producing bacteria from poultry waste, investigating keratinase enzyme produced by bacteria and understanding the conditions for optimal recovery of keratinase producing bacteria.Mixed design was used, following experimental procedures, entailing screening of isolates, formation of zone of clearance on skimmed milk agar and then evaluation for keratinase production, using raw feathers as substrate in submerged fermentation. Bacteria that produced the highest enzyme was selected for optimization of keratinase production. Different growth conditions were optimized to obtain maximum enzyme yield. Ten days of incubation period, Temperature of 40℃, pH 8, inoculum concentration of 12.50ml and substrate concentration of 12.50g were the optimal condition for keratinase production. Industries are encouraged to implement optimal conditions in the production of keratinase to obtain maximum productivity.
Full-Text Access Notice
In accordance with the NERD Policy on promoting peer-reviewed publication, public access to the full text of a project, thesis or dissertation is restricted for three years, allowing the author and supervisors sufficient time to pursue peer-reviewed publication.
During this period, researchers with legitimate academic or research purposes may request authorisation directly from the author to enable NERD to release the indexed full texts of the work using the form below.