ASSESSMENT OF BREWERIES WASTEWATER EFFLUENT EFFECT ON THE GROWTH AND PHYSIOLOGY OF GLYCINE MAX (L.) MERR. AND AMELIORATION USING BIOCHAR
Abstract
Beer production has promulgated the prevalence of heavy metal contamination in the environment (soil and water bodies). The magnitude of breweries wastewater-effluent effect discharged on soil and its effect on crop quality in terms of growth and physiology must therefore be investigated. This study aims to assess the level of seven heavy metals (HM) (lead (Pb), nickel (Ni), chromium (Cr), copper (Cu), cadmium (Cd), zinc (Zn) and iron (Fe)) in breweries wastewater effluent and its effect on the growth of Glycine max accessions (TGM-3990, TGM-1348, TGM-1732, TGM-2175 and TGM-928) as well as the influence of biochar soil amendment in the induction of HM tolerance in G. max. Effluent properties were analysed using the A1 portable TDS/EC meter. HM in the wastewater-effluent was determined using Atomic Absorption Spectrometer (AAS). The experiment was set up in a complete block design. 3 seeds G. max accessions were planted in 7kg of sterilized soil (control, effluent and biochar treatments). Growth parameters were taken using standard methods, while the total photosynthetic pigments (TPP) were determined using the atLeaf chlorophyll meter. The wastewater-effluent recorded an electrical conductivity (EC) of 928µS/cm, total dissolved solids (TDS) (464ppm), Temperature (31.9-32.6°C) and pH (5.37). Results for HM concentration showed; Pb (0.20mg/L), Ni (
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