THE IMPACT OF HONEY AND GRAPEFRUIT JUICE IN METHOTREXATE INDUCED TOXICITY IN SERUM LIVER ENZYMES IN FEMALE RATS
Abstract
Methotrexate (MTX) is a widely used chemotherapeutic agent known for it efficacy against various malignancies. However, its clinical utility is limited by its potential to induce hepatotoxicity, characterized by elevated serum liver enzymes levels. This study investigates the potential ameliorative effects of natural compounds, honey and grapefruit juice, in mitigating MTX –induced liver enzymes toxicity in female rats. Female Sprague-dawley rats were divided into five groups, Control, MTX, MTX with honey supplementation, and MTX with grape juice supplementation and grapefruit juice. Animal in the experimental groups received MTX (20 mg/kg) through interperitoneal injection. Honey was administered orally at a dose of 1g/kg, while grapefruit juice was given at 2ml/kg. Treatment regimens were carried out for 14 consecutive days. Serum samples were collected at the end of the treatment period, and liver enzyme levels including alanine transaminase (ALT), aspatate transaminase (AST), alkaline phosphatase (ALP) were assessed. Additionally, histopathological examinations of liver tissues were performed to evaluate morphological alterations.
Result revealed a significant elevation in serum ALT, AST, and ALP levels in the MTX group compared to the control. Conversely, rats supplemented with honey and grapefruit juice exhibited is a notable reduction in these enzyme levels, indicating a potential protective effect against MTX – induced hepatotoxicity. Histological analysis corroborated these findings, illustrating improved hepatic architecture in the supplemented groups. The present study demonstrated that both honey and grapefruit juice possess against MTX-induced liver enzymes toxicity in female rats. These findings suggest a promising avenue for the adjunctive therapy in patients undergoing MTX-based chemotherapy, potentially enhancing treatment tolerability and overall clinical outcomes. Further investigation are warranted to elucidate the underlying mechanisms and to validate these results in human cohorts
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