EVALUATION OF THE ADMINISTRATION OF TEA PREPARED FROM COLORADO ON REPRODUCTIVE SYSTEM OF FEMALE SPRAGUE-DAWLEY RATS

ABSTRACT The female reproductive system is highly sensitive to external substances, including herbal preparations and synthetic cannabinoids. This study evaluated the effects of tea prepared from dried synthetic marijuana (“Colorado”) on the reproductive system of adult female Sprague-Dawley rats. Twenty-five rats were divided into four groups: group A-control, group B- 1.5 mg/kg of Colorado, group C- 3.0 mg/kg of Colorado, and group D- 6.0 mg/kg of Colorado, each receiving treatment for 21 days. Body weight, estrous cycle patterns, reproductive hormone levels (FSH, LH, and estrogen), and histological architecture of the ovaries and uterus were assessed. Results revealed a decrease in the body weight of rats administered Colorado, which was dose-dependent. Treated groups exhibited irregularities in the estrous cycle. There was an increase in FSH, LH, and estrogen levels in colorado groups. There was histopathological changes in ovarian follicles and uterine tissues compared to the control group. These findings suggest that consumption of colorado exerts disruptive effects on female reproductive. The study highlights the reproductive risks associated with colorado and underscores the need for stricter regulation and public health awareness.