ANTIMICROBIAL EFFECTS OF NATURAL HONEY ON STAPHYLOCOCCUS AUREUS
Abstract
Medicinal property and antibacterial activity of honey on Staphylococcus aureus
and E. coli spp was studied. Fresh honey was bought from the market, then
phytochemcial analysis of honey was carried out for screening and identification of
secondary metabolites. The test isolates were confirmed using biochemical test and
then antimicrobial activity of honey was determined using agar well diffusion
method. The phytochemical analysis of honey indicates the presence of steroid
alkaloid and tannins while saponin, flavonoid and resin are absent. The honey
demonstrated antimicrobial activity against E. coli with the highest zone of
inhibition of (5mm), followed by Staphylococcus aureus (4mm) and lowest zone
of inhibition was observed in Salmonella spp (3mm) at 50mg/ml respectively. No
activity was observed at 1.562mg/ml. Based on the study it was concluded that
honey is active against the bacterial isolates and can be used as a therapeutic
substances for bacterial infection
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