GC–MS ANALYSIS AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL FROM THE LEAVES OF BRYOPHYLUM PINNATUM
Abstract
Bryophyllum pinnatum is a medicinal plant traditionally used for the treatment of wounds,
inflammation, and oxidative stress–related conditions. In this study, the phytochemical composition of
its leaves was investigated using Gas Chromatography–Mass Spectrometry (GC–MS) and the
antioxidant potential was assessed through DPPH, ABTS, and FRAP assays. GC–MS analysis revealed
methoxy-phenyl (2.83%),Allydimethyl silane (2.13%), phenol (6.16), benzoic acid,methyl ester
(20.31%), all associated with antioxidant activity. Antioxidant assays showed significant variation with
extraction method. Hydrodistillation (HD) (Essential oil) exhibited a higher reducing power in the
FRAP assay (19.2) compared to Cold Extraction (CE) (Bioactive) (3.32), and also demonstrated
stronger radical scavenging in the ABTS assay (33%) relative to CE (18.6%). In contrast, CE showed
greater activity in the DPPH assay (18%) compared to HD (2%), reflecting its higher content of
phenolic antioxidants. These results indicate that Bryophyllum pinnatum possesses a dual antioxidant
system, with CE favoring phenolic-based antioxidants and HD concentrating volatile and lipophilic
compounds. The findings provide scientific support for the traditional use of Bryophyllum pinnatum
and highlight its potential as a natural source of antioxidants.
Full-Text Access Notice
In accordance with the NERD Policy on promoting peer-reviewed publication, public access to the full text of a project, thesis or dissertation is restricted for three years, allowing the author and supervisors sufficient time to pursue peer-reviewed publication.
During this period, researchers with legitimate academic or research purposes may request authorisation directly from the author to enable NERD to release the indexed full texts of the work using the form below.