ISOLATION AND IDENTIFICATION OF MICROORGANISMS FROM FRIED FISH SOLD WITHIN MAKURDI.

Student: John Ikpa Ogbanje
Supervisor: Dr S B Valdo
HOD: Dr Garba Thadime Appolos
Department of Biological Sciences
Science
Rev. Fr. Moses Orshio Adasu University, Makurdi

Abstract

Fisharecooked,friedandeatenfreshormoreoften,driedinthesunafterremovalof theirgutsandscalesandsometimestheyaresmoked.Thisstudyevaluatesthe microorganismsassociatedwithfriedicefishsoldwithinMakurdi.Friedicefish sampleswereprocuredfrom Wurukum market,Wadatamarket,Modernmarketand High levelmarkets within Makurdi,Samples were analyzed using standard microbiol ogicaltechniques.The isolates were identified using morphological characteri sticsandbiochemicaltests.Alldatacollectedwasanalyzedusingthe Statisti calPackageforSocialSciencesversion18(PASW statistics18).Theresult showedatotalofsevendifferentbacteri alspecieswhichinclude:Sal monellasp.which wasobservedasthemostoccurringbacteriumwiththeoccurrenceof45representing 34.62%ofthetotaloccurrenceobserved.Thiswasfoll owedbyStaphylococcusaureus withtheoccurrenceof42(32.31%),Klebsiellasp.18(13.85%),E.coli14(10.77%), CoagulasenegativeStaphyl ococcus6(4.62%),Pseudomonassp.4(3.08%)andS.typhi whichhastheleastoccurrenceof1(0.77%).Itwasobservedthatthefriedicefish sampledfromWadatahadthehighestbacterialoccurrenceof52(40.00%),fol lowedby thosefromWurukum30(23.08%).ThesamplesfromHighLevelandNorthBankwere observedtohaveequalbacterialoccurrenceof24(18.46)each.Salmonellasp,S. aureusandKlebsiell asp.wereobservedinallthelocati ons.Theoccurrenceofthefungi associatedwithfriedicefishwasalsoexamined.Atotalfivefungalspecieswere observedwhichwere;Mucorsp.whichhasthehighestoccurrenceof22(23.91%), Aspergil lusnigerwhichhas21(22.83%),A.Flavus19(20.65%),Candi dasp.17918.48%) andPenicill iumsp.whichhastheleastoccurrenceof13(14.13%).Thisstudyrevealed ahighlevelofmicrobialcontaminati oninfriedicefishsoldwithi nMakurdimetropolis, withsevenbacterialspecies(l edbySalmonellasp.)andfivefungalspecies(ledby Mucorsp.)isolated.Thecontaminati onwaswidespreadacrossdifferentlocati ons,with Wadata and Wurukum showing the highestbacterialand fungaloccurrences, respecti vely.Thepresenceofpathogenicmicroorganisms,incl andAspergillusflavus,posesasignificantri udingSalmonellatyphi sktopublichealth.Therefore,urgent attenti onisneededtoimprovehandling,storage,andhygienepracti cesthroughoutthe foodchaintominimizetheriskofmicrobi alcontaminati onandensurethesafetyofthis popular food product.

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