WASTE WATER PURIFICATION THROUGH ADSORPTION PROCESS
Abstract
Staphylococcus aureus is a prevalent skin commensal and opportunistic pathogen, necessitating effective hand hygiene practices to prevent infections. This study aimed to isolate and identify S. aureus from palm samples of volunteers and evaluate the antibacterial efficacy of five commercially available detergents (labeled A, B, C, D, and E) against the isolated strains. Palm swabs from 50 healthy volunteers were cultured on Mannitol Salt Agar, with 84% of samples confirming S. aureus through Gram staining, catalase, and coagulase tests. The disk diffusion method revealed that Detergent D produced the largest zones of inhibition (up to 21.5 mm at 50% concentration), followed by Detergent A, while Detergent C showed the least activity. Minimum inhibitory concentration (MIC) assays further confirmed Detergent D’s superior potency, with an MIC of 1.25% (v/v) compared to 7.5% for Detergent C. Statistical analysis indicated significant differences in efficacy (p < 0.05). These findings highlight Detergent D’s potential for effective hand hygiene and underscore the need for further research into optimizing detergent formulations to combat S. aureus while ensuring skin safety.
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