ISOLATION, CHARACTERIZATION, AND ASSESSMENT OF BIOFILM-FORMING POTENTIAL OF BACTERIA FROM UNDERGROUND STORED WATER

Although it is essential to have access to clean water, many people still use sources that are susceptible to microbial contamination. This study examined the internal tap surfaces and tap water microbiological quality in student residences(Purpose and Peace hostels) at Benson Idahosa University in Edo State, Nigeria. Antibiotic resistance, biofilm forming capability, species identification, and bacterial load were all examined in samples taken from the Peace and Purpose hostels. A total of ten samples were analyzed. Results showed high heterotrophic, and coliform counts up to 5.88 CFU/ml and 3.69 CFU/ml respectively indicating significant microbial contamination. The findings demonstrated the existence of dangerous bacteria, such as Proteus spp, Pseudomonas aeruginosa, and Staphylococcus aureus, several of which exhibited high resistance to widely used antibiotics like Augmentin and Cefotaxime. High concentrations of heterotrophic bacteria and coliforms point to inadequate water hygienic practices and biofilm development in distribution and storage networks. Particularly for students who use this water on a daily basis for drinking and bathing, the findings pose public health concerns. This study emphasizes the critical need for stricter antibiotic stewardship, better sanitation procedures, and enhanced water quality monitoring in shared living settings.