INVESTIGATION INTO THE MICROORGANISMS ASSOCIATED WITH SPOILAGE OF CANNED TOMATOES (CLAPPA AND SONIA)

Student: Ngozi Gift Oko
Supervisor: Mr E Asogwa
HOD: Mr M U Agama
Department of Microbiology
Science and Laboratory Technology
Institute of Management and Technology, Enugu, Enugu State

Abstract

Investigation into the Microorganisms Associated with Spoilage of Canned Tomatoes (Clappa and Sonia); pour plate technique was employed for isolation using nutrient agar, MacConkey agar and Sabround agar. Also biochemical tests were carried out to differentiate organisms into different morphology and characteristics as well as gram staining. The growth pattern, pigmentation and size of colonies were recorded at the incubation period to aid identification of the organisms. The absence of blown and leaky cans could suggest that all the samples analysed within expiry date from manufacture is of acceptable quality for consumption. The canned foods examined had low microbial loads < 106 within acceptable microbiological quality. The combined effects of high temperature treatment, pH, preservatives and anaerobic condition of canning could have been responsible for the low microbial loads. Tomatoes and Sardines had higher plate counts; these could be a reflection of the quality of the raw materials, under processing, pre-process contamination and to the level of stringency in their production

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