NEMATICIDAL POTENIAL OF LEAVES EXTRACTS OF THAUMATOCOCCUS DANIELLII ON MELOIDOGYNE INCOGNITA
Abstract
ABSTRACT
This study investigates the phytochemical composition and nematicidal properties of leaf extracts from Thaumacoccus danielli, a plant known for its medicinal and agricultural benefits.Using various extraction methods (aqueous, ethanolic and methanolic), the study identified key phytochemicals, including flavonoids, alkaloids, saponins and tannins through standard qualitative tests.
In vitro assays demonstrated that T. danielli leaf extracts significantly affected the viability of Meloijdogyne incognita, a major pest in agricultural systems. The methanolic extract exhibited the highest nematicidal activity, reducing juvenile nematode mortality by over 80% within 48hours. Additionally, histopathological analysis indicated morphological alterations in nematodes exposed to the extracts, suggesting potential mechanisms of toxicity.
These findings support the use of Thaumatococcus danielli as a sustainable alternative for managing root-knot nematodes, offering a promising avenue for integated pest management strategies in agriculture. Further research is recommended to isolation and characterize the specific phytochemicals responsible for nematicid activity and to evaluate their potential in field conditions.
The Aim of this study is to investigate fumigant toxicity of Datura metel extraion Callosobruchus maculatus. Datura Metel leaf powder was extracted with hexane, ethyl acetate, acetone, chloroform, and methanol and tested at 50, 100 and 150 ul concentrations. Adult mortality, oviposition different, infestation, tolerance, weight loss and germination were calculated and analysed using statistical tool (ANOVA). At 24h exposure, ethyl acetate had the highest significant mortality (50.75%) and (89.67% respectively) with the least LC50 (0.03ul) recorderd at 72h. Per cent egg hatch ability was highest, in control (93.33%), lowest in ethy acetate (8.33%) at. 150ul concentrations and had highest reduction in adult emergence at 50(55.38%), 100(86.67%), and 150ul(93.
33%) respectively. There were 100% tolerance in the control and 63.60-75.55% tolerance at 150ul, in treated dishes resulting in significantly (p
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