PHYTOCHEMICAL SCREENING, INVITRO AND INSILICO STUDIES, ANTI FUNGAL AND ANTI BACTERIA ACTIVITIES FROM EXTRACT OF KIGELIA AFRICANA

The use of herbal medicines and phytonutrients with numerous secondary metabolites continues to expand rapidly across the world, with many people now resorting to these products to treat various health challenges in different national healthcare settings. Therefore, the study is aimed at evaluating the phytochemical composition in the fruit peel extract of Kigelia africana, to ascertain its numerous pharmacological activities and identify the various chemical compounds responsible for these activities. Molecular docking is a computational technique used to predict the binding affinity and orientation of small molecules (ligands) within a protein’s active site. When combined with Prediction of Activity Spectra for Substances (PASS) and Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) predictions, molecular docking provides a comprehensive approach to drug discovery and development. Dichloromethane, ethylacetate and methanol extracts of identified fruits peel of K. africana stalk were prepared for phytochemical screening using established methods for Alkaloids, Tannins, Phenols, Flavonoids, and Terpenoids testing etc. The study also investigated the antimicrobial potential of our samples against various Fungi and Bacterial pathogens. The Mycelial inhibition method was employed to access antifungal activity, measuring the inhibition of fungal growth in the presence of the test substance. The agar well diffusion method was used to evaluate antimicrobial activity, determining the zone of inhibition around wells containing the test substance.