ASSESSMENT OF ANTIOXIDANTS ACTIVITY OF CONOCLINIUM COELESTINUM LEAF EXTRACTS AGAINST INDOMETHACIN INDUCED RATS.
Abstract
Indomethacin, a non-steroidal anti-inflammatory drug, is known to induce oxidative stress by
generating reactive oxygen species. The study was aimed to assess the antioxidant activity of
Conoclinium coelestinum leaf extract in mitigating oxidative stress induced by indomethacin in
rats.The effects of prophylactic treatment with C. coelestinum extract on catalase, superoxide
dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx), and
malondialdehyde (MDA) concentrations in rats were determined . Rats were divided into groups:
control, indomethacin-treated, and prophylactically treated with C. coelestinum extract before
indomethacin induction. Catalase, SOD, GSH, GPx, and MDA levels were assessed using standard
biochemical assays. Results showed that indomethacin significantly reduced catalase, SOD, GSH,
and GPx activities while increasing MDA levels, indicating oxidative stress induction. In contrast,
prophylactic treatment with C. coelestinum extract mitigated these effects, significantly increasing
catalase, SOD, GSH, and GPx activities, and decreasing MDA levels compared to the
indomethacin-only groupThese findings suggest that C. coelestinum leaf extract exhibits potent
antioxidant properties, protecting against indomethacin-induced oxidative damage by enhancing
antioxidant enzyme activities and reducing lipid peroxidation. The study shows the therapeutic
potential of C. coelestinum as a natural antioxidant agent in oxidative stress-related conditions,
potentially offering novel avenues for preventive and therapeutic strategies in inflammatory
disorders
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