ASSESSMENT OF OXIDATIVE STRESS IN INDIVIDUALS WITH TUBERCULOSIS IN OWO METROPOLIS

ABSTRACT Oxidative stress play an important role in the pathogenesis of tuberculosis. To our knowledge there is paucity of data on the status of oxidative stress biomarkers among TB patients. This study was designed to evaluate the oxidative stress biomarkers in tuberculosis patients. The aim and objectives of the study was to determine the serum levels of the oxidative stress biomarkers among tuberculosis patients in Owo metropolis, Ondo state and to assess the correlation between the oxidative stress biomarkers in tuberculosis patients. A cross sectional comparative study was conducted on Thirty (32) tuberculosis subjects and Twenty (20) age and sex-matched controls who were recruited into this case-control study from General Hospital, Owo. 5 ml of venous blood sample was collected into plain vacutainer blood sample bottle and allowed to clot and later centrifuged at 5000 rpm for 10 minutes and the serum was removed to another plain tube and stored until the time of analysis, sample showing hemolysis was discarded.. Serum total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), nitric oxide (NO) were determined using standard techniques. Data was analysed using Instat® . Serum levels of TOS, MDA and NO were significantly increased in Mycobacterium tuberculosis patients when compared with healthy individuals. .Serum TAS was significantly decreased in Mycobacterium tuberculosis patients compared with healthy individuals. Serum TOS significantly correlated with serum TAS MDA, and NO. This study observed an increased oxidative stress biomarkers and decreased total antioxidant status in Mycobacterium tuberculosis patients. Our findings suggests that antioxidants supplementation and improved nutrition in the management of Mycobacterium tuberculosis patients may go a long way in preventing the oxidative onslaught and further complications in Mycobacterium tuberculosis patients.