DETECTION OF ANTIBIOTICS RESIDUES AND TETRACYCLINE RESISTANCE BACTERIA ISOLATES IN POULTRY WASTEWATER
Abstract
Background and Aim: Antibiotic residues in poultry farming have been identified as a potential cause of antimicrobial resistance in humans and animals. This study aimed to determine antibiotic residues present in poultry waste water and level of resistance.
Materials and Methods: To detect antibiotic residues in the collected samples using UV spectrometer. Morphological and biochemical characteristics of the isolates were determined and susceptibility test was carried out to determine the level of antibiotic resistance. A total of 5 samples were analyzed for antibiotic residues of commonly used 11 antibiotics. Out of these, 2 samples were collected from broilers, 1 from ostritch, 1 from qwells and1from duck. UV spectromether was used to detect the presence of residues from various poultry waste water sample (Chicken, Duck, Qwels, and Ostritch).
Results: UV spectrometer analysis revealed that 23.5% of the tested samples contained residues from six different antibiotic classes (tetracyclines, quinolones, beta-lactams, sulfonamides, aminoglycosides, and macrolides). The result of the study revealed that, of the 5 samples tested, all were positive to the presence of antibiotic residues in the poultry waste water samples. Morphological and biochemical characteristics corfirms the presence of Micrococcus luteus, Aeromonas hydrophile, Pasteurella matocida, Proteus mirabilis, Proteus mirabilis and Proteus vulgaris. Susceptibility test of the samples revealed that resistance was observed in the tested antibiotics towards all the samples except for susceptibility in Nalidixic acid and Cephalexin.
Conclusion: This study clearly showed the misuse of antibiotics in the poultry sector within the studied area. And the antimicrobial resistance shown by the isolated pathogens possess a serious hazard to human life.
Keywords: antibiotic residues, UV spectrometer, susceptibility, Biochemical, Morphological.
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