EFFECTS OF DIALLYL DISULFIDE ON MERCURY CHLORIDE INDUCED HEPATOTOXICITY IN MALE WISTAR RATS.
Abstract
Oxidative stress and cellular dysfunction are the main causes of the hepatotoxic effects of
mercury chloride (HgCl₂), a well-established environmental toxin. Even though diallyl
disulfide has been the subject of liver and renal studies, more research is still needed. In order
to evaluate the biochemical indicators of liver function enzymes, this study examined the
protective effects of diallyl disulfide (DADS) against HgCl₂-induced liver damage in male
Wistar rats. An experimental strategy was applied. Twenty-eight adult male Wistar rats
weighing 130–160 grams were split into four groups at random: DADS (40 mg/kg), HgCl₂ (6
mg/kg), Control (normal saline and olive oil), and DADS + HgCl₂ (40 mg/kg and 6 mg/kg).
The Wistar rats were administered toxicant and treatment for 14 days before they were
sacrificed to collect and analyse liver tissues. Biochemical assays were conducted to measure
liver function markers, including Alanine Aminotransferase (ALT), Aspartate
Aminotransferase (AST), and Alkaline Phosphatase (ALP). Lipid peroxidation was assessed
by determining the levels of Malondialdehyde (MDA), a key biomarker, while antioxidant
defense was evaluated through the activities of Glutathione (GSH), Glutathione Peroxidase
(GPx), Catalase, and Superoxide Dismutase (SOD). HgCl₂ exposure significantly elevated the
levels of ALT, AST, and ALP, indicating substantial liver damage. MDA levels significantly
increased, indicating increased oxidative stress and lipid peroxidation. Male Wistar Rats'
hepatotoxic effects were considerably lessened by DADS treatment. In comparison to the
HgCl₂ group, DADS improved the antioxidant defense system and raised the activities of
GSH, GPx, Catalase, and SOD in the HgCl₂ + DADS group. According to the study's
findings, DADS may be a useful treatment for stopping and treating liver damage brought on
by mercury.
Keywords: Mercury chloride, Diallyl disulfide, Reactive oxygen species, Antioxidant
enzymes, Liver function biomarkers.
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