PHYTOCHEMICALS SCREENING AND ANTI INFLAMMATORY PROPERTIES OF MANGIFERA INDICA (MANGO) STEM BARK EXTRACT

The present study investigates the phytochemical composition and anti-inflammatory properties of Mangifera indica stem bark (Mango) , with a focus on its potential as a natural alternative to synthetic anti-inflammatory drugs. Both aqueous and Methanolic extracts of the plant were analyzed for phytochemical content and biological activities. The qualitative analysis revealed the presence of key phytochemicals such as tannins, flavonoids, saponins, steroids, terpenoids, and alkaloids, with ethanol proving to be a more effective solvent for most compounds, except for flavonoids, which were better extracted by water. Quantitative analysis showed that tannins (4.56%) and flavonoids (3.32%) were the major constituents, both of which are known for their antioxidant properties. The anti-inflammatory activities of Mangifera indica stem bark (Mango) were evaluated using different biological models, including heat-induced protein denaturation, membrane stabilization, and lipoxygenase inhibition. Results showed that Mangifera indica stem bark (Mango) exhibited dose-dependent anti-inflammatory effects in all assays. At concentrations ranging from 10 μg/ml to 50 μg/ml, the plant's extract provided significant protection against protein denaturation (50.3% to 66.8%), membrane stabilization (48.7% to 65.9%), and erythrocyte hemolysis (49.6% to 64.8%). Additionally, the plant exhibited notable inhibition of lipoxygenase activity, ranging from 52.4% at 10 μg/ml to 67.5% at 50 μg/ml. Despite these promising results, Mangifera indica stem bark (Mango) was found to be less potent than standard anti-inflammatory drugs like Diclofenac Sodium, However, the plant’s phytochemical-rich profile and its ability to exert significant biological effects justify its use in traditional medicine for managing inflammatory conditions. Further studies are recommended to explore the bioactive compounds in Mangifera indica stem bark (Mango) and their mechanisms of action, with the goal of developing natural anti-inflammatory therapies.