PHYTOTOXIC ACTIVITY AND SPECTROSCOPIC IDENTIFICATION OHROMATOGRAPHIC FRACTION OF LEAF EXTRACT OF MUSAACUMINATA
Abstract
Plantssynthesizehundredsofchemicalcompoundsforfunctionsincludingdefenseagainstinsect,fungi,
diseases,andherbivorousmammals.Phytotoxicityisthedetrimentaleffectofachemicalorsubstanceon
plantgrowth,development,orproductivity,resultinginalterationstoplantmorphology,physiology,orb
iochemistry.Theextensiveandunevenapplicationofsyntheticherbicideshascausedsevereenvironme
ntalpollution,humanhealthproblems,anddestructionofbiodiversity.Thisresearchisthereforeaimedto
evaluatechemicalsandphytotoxicpotentialofMusaacuminataleavesextract.
FreshleavesofMusaacuminatawerecollected,washedandauthenticated.50gofthepowderymaterialw
assoakedinaqueousandorganicsolventsina1:5solid-tovolumeratioandsonicatedfor15mininanultrasonicbath.Concentrationsof0.25,0.5,0.75,and1%(v/v)
wereusedforthephytotoxicitywhiledistilledwaterandwaterethanolmixtureswereusedasthecontrolfortherespectivesolvents.Thephytotoxicpotentialwastesteda
gainstM.sylvestris,C.album,andL.sativumseedsandseedlings.Columnwaspackedwithsilicagelandth
eeluentwassubjectedtoUVanalysisandFTIR
Resultsindicatedthatthegerminationpercentageofseedswasreducedsignificantly(p>0.05)inresponse
toalmostallextracts,ascomparedtothecontrol.thefirstthreeconcentrationsreducedthegerminationper
centagetozeroandupto95%decreasewasobservedinMsylverisandC.albumrespectively.Adosedependentrelationshipwasobservedinregardstotheinhibitoryimpactandelongationofradicleandstem
.GCMSresultsrevealedpresenceofcompoundssuchas-anthracenyltrimethylsilane,4’-
dimethylamino-2’-(trimethylsilyl)acetanilide,2-Acetyl-3-methylaminocyclopentenone,5-Methyl-
4-hydroxybenzoylhydrazonefurfurol,4-(3‚4-dimethoxyphenyl)-5-methyl-2-
thiazolamine,(Chloroaceticacid),whiletheIRshowedN-Hstretch,C-Hasymmetricstretch,Cvi
Hsymmetricstretch,C-Hstretch,S-HstretchandHNCSstretches.Confirmingpresenceofdifferentaromaticgroups
TheresearchconcludedthatMusaacuminataleavesextracthavephytotoxiceffectagainsttestedplantsa
ndimportantphytochemicalswerepresentintheextract.Itisrecommendedthattheplantextractbemaxi
mizedinproductionofherbicidesandfurtherstudiesbedonetoestablishitsphytotoxiceffectsonotherpla
nts.
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