ISOLATION AND IDENTIFICATION OF FUNGI PRODUCING LIPASES FROM SOIL IN REFUSE DUMP SITE IN ISA MUSTAPHA AGWAI I POLYTECHNIC LAFIA

The project topic “Isolation And Identification Of Fungi Producing Lipases From Soil In Refuse Dump Site In Isa Mustapha Agwai 1 Polytechnic Lafia”. Is aimed at isolating and identifying fungi producing lipases from soil samples collected around Isa Mustapha Agwai I Polytechnic, Lafia. The following materials and methods were used in carrying out this research such as Soil sample, sterile gloves, petri dishes, conical flask, etc using the serial dilution method. The result gotten shows that sample A, C and E growth rate was slow with black, green and yellow pigment respectively and Fungi isolated were Aspergillus niger, Penicillum halicum and Aspergillus plavus respectively while the sample B and D has the growth rate fast and moderate respectively and the fungi isolated were Mucor spp and Trichophton terrestre respectively.. Lipases are important enzymes with diverse applications in industries such as biodiesel production, food processing, and bioremediation. Soil samples were systematically collected from three distinct locations within the refuse dump site, and fungal strains were isolated using selective culture media and incubation techniques that promote fungal growth. This study highlights the potential for utilizing indigenous fungi from waste environments in biotechnological applications and contributes to the understanding of microbial diversity in refuse dump sites. Therefore, it is recommended that non- lipases producing fungi should be investigated and the biotechnological application should be applied. Keywords: Fungi, Lipase, Isolation, Refuse Dump Site,