IN VITRO SCREENING OF RED VELVET SOLUTION FOR ANTIOXIDANT CAPACITY AND SECONDARY METABOLITES

This project explore the antioxidant capacity and secondary metabolites of synthetic red velvet colourant solution. Through the assays such a DPPH test, lipid peroxidation, Total flavonoid content test, the study assesses the colourant ability to neutralize free radicals, revealing notable antioxidant properties was carried out. Additionally the study also identified secondary metabolites that contribute to the choice of red velvet in consumption, Secondary metabolites were phenol, tannin, saponin, cardiac glycosides, triterpenoids, alkaloids and flavonoid were not present in the solution of red velvet. The red velvet colourant give limited antioxidant, the result obtained throuh the assays such as DPPH, of different concentrations, 100µl, 200µl, 300µl, 400µl, 500µl, 600µl and700µl with percentage results of-138.5,-159.9,-163.9,-159.5,-165.4,-140.6,-166.5, respectively, Lipid peroxidation inhibition of different concentrations, of 50µl, to 450µl with percentage results 0.007,0.005,0.005,0.004,0.004,0.003,0.003,0.003, respectively, Hydroxyl free radical inhibition power of red velvet solution of different concentrations, 50µl, 100µl, 150µl, 200µl, 250µl, 300µl, 350µl, 400µl, 450µl with percentage results 73.97,61.76,47.65, 30.23,25.23,-12.01,-8.61,-12.51, 23.82 respectively. The finding showed that the lower the concentrations of the synthetic red velvet colourant exhibit better antixdant power.The overall levels of secondary metabolites are low, their potential efficacy may enhanced at reduced concentration. For DPPH assay the colourant do not exibit scavenging power, and Hydroxyl free radical inhibition power the colourant inhibit from the lower concentration which is 50µl,to 250µl, from 300µl, to450µl, it does not inhibit free radical