ISOLATION AND IDENTIFICATION OF MICROORGANISMS FROM PEELED GROUNDNUT SOLD IN OWERRI METROPOLIS

Student: Mary Nkorika Anyalebechi
Supervisor: Dr I J Ibe
HOD: Mrs A. K. Osikwe
Department of Microbiology
School of industrial and applied sciences
Federal Polytechnic, Nekede, Imo State

Abstract

Isolation and identification of microorganisms from peeled groundnut sold in Owerri metropolis was investigated. A total of ten (10) groundnuts peeled were randomly purchased from ten (10) different vendors within Owerri metropolis, Imo State, Nigeria, Standard microbiological plate count was used to isolate the microorganisms. Total viable bacterial counts recorded ranged from 5.0 x 10 cfu/g to 1.4 x 10'cfisg. Total coliform counts recorded ranged from 1.0 10 cfu/g to 2.0 x 10 cfu/g while total fungal counts recorded ranged from 6.0 x 10 cfu/g to 5.0 x 10fcfu/g. There were no coliform counts recorded with seven (7) out of the ten (10) groundnut samples used in this study. Bacterial isolates, frequency and percentage occurrence recorded include; Preudomonas 9(34.6%), Bacillus 10(38.5%), Escherichia 2(7.6%), Micrococcus 1(3.9%), Streptococcus 1(3.9%) and Staphylococcus species 3(11.5%). Fungal isolates, frequency and percentage occurrence recorded include; Mucor 7(35.0%), Rhizopus 6(30.0%) and Penicillium species 7(35.0%). Considering the importance of groundnut as a poor man's nut, good quality control practices should be encouraged among the vendors.

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