ANTIBIOTICS SUSCEPTIBILITY PATTERN OF BACTERIA ISOLATED FROM CLINICAL SAMPLE

ABSTRACT The emergence of antimicrobial resistance among clinical bacterial isolates poses a significant threat to public health globally. This study investigated the antibiotic susceptibility profiles of Escherichia coli and Staphylococcus aureus isolated from clinical specimens using the disk diffusion method, and results were interpreted based on Clinical and Laboratory Standards Institute (CLSI) guidelines. The isolates were tested against a panel of commonly prescribed antibiotics including Gentamicin, Erythromycin, Ciprofloxacin, Augmentin, Pefloxacin, Ampiclox, Zinnacef, Amoxicillin, Rocephin, Streptomycin, Septrin, Chloramphenicol, Tarivid, and Sparfloxacin. E. coli showed high susceptibility to Septrin (27.0 mm), Augmentin (26.0 mm), Chloramphenicol (25.0 mm), Tarivid (23.0 mm), and Streptomycin (22.0 mm), with resistance to Gentamicin, Erythromycin, and Sparfloxacin. S. aureus displayed strong sensitivity to Ciprofloxacin (32.0 mm), Septrin (20.0 mm) and Tarivid (21.0 mm), while being resistant to multiple β-lactam antibiotics including Gentamicin, Amoxicillin, Zinnacef, Rocephin, and Ampiclox. The observed resistance patterns highlight a growing incidence of multidrug-resistant strains, likely due to overuse and misuse of antibiotics. This study emphasizes the urgent need for antibiotic stewardship, routine susceptibility testing, and public health awareness campaigns to mitigate the spread of resistant pathogens and ensure effective clinical management of infections.