GREEN SYNTHESIS, CHARACTERIZATION AND ANTI MICROBIAL ACTIVITIES OF NANO PARTICLES OF MUSA ACUMINATA SILVER NANO PARTICLES

Student: Peace Dominic Ayang
Supervisor: Dr Moses Edim Mbeh
HOD: Dr Patience Yaka Nkom
Department of Chemistry
Physical Sciences
University of Cross River State, Calabar

Abstract

ABSTRACT This study, silver nanoparticles (AgNPs) were synthesized via a green approach using Musa Acuminata extract as a reducing and stabilizing agent. Characterization was performed using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX) analysis. FTIR spectra revealed characteristic bands corresponding to hydroxyl (O- H), carbonyl C=O 1643.28 cm-1), and amine (N-H) functional groups with additional peaks observed at 802.68, 883.64, 1307.57, 2819.83, 559.62 cm-1, confirming the role of Musa acuminata. Phytochemicals such as phenolics, flavonoids, and tannins in reducing Ag+ to Ag0 and stabilizing the nanoparticles. SEM micrographs showed a highly porous, irregular fibrous surface, providing numerous active sites for nanoparticle nucleation. EDX analysis further confirmed nanoparticle formation, with silver detected as the most abundant element (29.12% atomic concentration), alongside carbon, iron, zinc, and other trace minerals. Antimicrobial activity, assessed by the agar well diffusion method, showed dose-dependent inhibition against Escherichia coli. (11mm at 50 mg/L) showed to be most abundant. These results demonstrate that Musa acuminata- derived AgNPs are an eco-friendly, cost-effective material with significant antimicrobial potential. This abstract describes the synthesis and characterization of silver nanoparticles using a plant extract, and it highlights their potential as an antimicrobial agent.

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