ISOLATION OF CARBAPENAMASE PRODUCING UROPATHOGENIC PSEUDOMONAS AERUGINOSA FROM PREGNANT WOMEN ATTENDING TERTIARY HOSPITAL IN RIVERS STATE
Abstract
ABSTRACT
P. aeruginosa poses a significant threat to healthcare settings, particularly among vulnerable populations like pregnant women.
To isolate carbapenamase-producing Pseudomonas aeruginosa from the urine of pregnant women attending Tertiary Hospitals in Rivers State, Nigeria.
Urine samples were collected from consenting pregnant women attending antenatal clinics of the tertiary hospitals in Rivers state (UPTH, RSUTH, and MAUTH) and processed for bacterial culture. The isolates were evaluated using different biochemical methods. The antimicrobial susceptibility testing of the isolates was determined using the disc diffusion technique. The Carbapenem resistance characteristics of the isolates were determined phenotypically using imipenem discs. The carbapenamase production was carried out using the Modified Hodges test and the Triton Hodges test.
A total of 423 urine samples were collected, with the number of isolates as follows; 2 (1.19%) for UPTH, 6 (3.87%) at RSUTH, and 0 (0%) at MAUTH for p.aeruginosa. From the study, resistance to antibiotics, such as Amoxicillin clavulanate Cefotaxime, Imipenem, Nalidixic acid, Cefuroxime, Ampiclox, Levofloxacin (100% resistance at UPTH) and Amoxicillin clavulanate, Imipenem, Nitrofurantoin, Cefuroxime, Ampiclox, Cefexime (100% at RUSTH) was recorded. Overall effectiveness of antibiotics was low, making treatment difficult. There was a notably high prevalence of carbapenem resistance, with resistance patterns showing serious multidrug resistance in all isolates. None of the isolates produced carbapenamases.
There is a low significant presence of P.aeruginosa infection among pregnant women in hospitals in Rivers State, and they also show high levels of multidrug resistance. We urgently need better infection control measures and responsible antibiotic use that fit the specific resistance patterns seen in different healthcare settings.
P. aeruginosa, carbapenemase-producing Pseudomonas aeruginosa, multi-drug resistance, antibiotic susceptibility, modified Hodges test, triton hodges test.
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