ISOLATION AND IDENTIFICATION OF BACTERIA FROM URINARY TRACT INFECTIONS (UTIS) IN PETIENT ATTENDING GENERAL HOSPITAL AZARE

ABSTRACT This study investigates the isolation and identification of bacteria urinary tract infections(UTIS) at attending general hospital Azare Bauchi, Nigeria. A cross-sectional study was conducted involving symptomatic patients with urinary tract infections (UTIs). Mid-stream urine samples were collected from pettient suspected having UTIs processed to identify bacterial species, from urine culture and isolated bacterial organism from urine sample organism identify bacterial isolated using gram staining and biochemical test determine their antibiotic susceptibility patterns, of the bacterial isolated. The isolation process utilized MacConkey agar and other selective media, with identification based on colony morphology, Gram staining, and biochemical tests. Results indicated that Escherichia coli was the most frequently isolated organism, accounting for 61.10% of cases, followed by Pseudomonas aeruginosa (20.00%), Proteus mirabilis (13.33%), and Klebsiella pneumoniae (5.56%). Antibiotic susceptibility testing revealed high sensitivity of E. coli to Ciprofloxacin (92.73%) and Gentamicin (90.91%), but significant resistance to Amoxicillin (5.45%). The study also identified a bacterial among isolates, with 25% of E. coli and 44.4% of P. aeruginosa classified as a antibiotics.These findings underscore the pressing issue of antibiotic resistance in the treatment of UTIs within the region and highlight the necessity for continuous surveillance and rational antibiotic use to mitigate resistance development.