COMBINED IN-VITRO EFFECTS OF AQUEOUS AND METHANOL EXTRACTS OF AZADIRACHTA INDICA AND CARICA PAPAYA LEAVE EXTRACTS AGAINST MULTI-DRUG RESISTANT DIARRHEAGENIC BACTERIA

ABSTRACT A major threat to global public health is the advent of microbes resistant to many drugs that cause diarrhea. The effectiveness of conventional treatment choices is declining, which makes the investigation of alternative therapeutic approaches necessary. The purpose of this study is to assess the antibacterial activity of Azadirachta indica (neem) and Carica papaya (pawpaw) separately and in combination against organisms that cause diarrhea that are resistant to multiple drugs. This research is aimed at evaluating the efficacy of Azadirachta indica (neem) and Carica papaya (paw paw) leave extracts against a panel of multi-drug resistant diarrhea causing organisms such as Salmonella typhi, Shigella dysenteriae, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli, which are frequently linked to infections related to diarrhoea. The application of these extracts resulted in growth inhibition and a decrease in bacteria viability, confirming the antibacterial characteristics indicated in the previously referenced study. Further boosting their antibacterial activity, this research showed that Azadirachta indica (neem) and Carica papaya (pawpaw) leave extracts combined had a synergistic impact. As an adjuvant or alternative treatment for multi-drug resistant diarrhea caused by pathogenic organisms, this research's findings indicate that the above-mentioned leave extracts have significant promise. With the potential to lessen reliance on conventional antibiotics, these leave extracts inherent antibacterial qualities present a viable approach to treating drug-resistant diarrhea pathogens. In order to treat multi-drug resistant diarrhea illnesses, the findings establish the foundation for future research into the creation of innovative therapeutic approaches.