ANTIMICROBIAL ACTIVITIES OF DELONIX REGIA AGAINST SOME PATHOGENIC ORGANISMS

An abstract for a laboratory-based microbiology and pharmacognosy thesis must clearly outline the research problem, the extraction process, the specific pathogens tested, key analytical results, and the clinical implications of the findings.Below is an academic abstract tailored for the Antimicrobial Activities of Delonix regia Against Some Pathogenic Organisms topic, formatted to standard institutional guidelines (between 200 and 300 words).ABSTRACTThe rapid acceleration of antimicrobial resistance (AMR) among human pathogens represents a critical global healthcare crisis, rendering many conventional synthetic antibiotics ineffective. This trajectory has necessitated an urgent exploration of plant-based antimicrobials as alternative therapeutic agents. This study evaluated the Antimicrobial Activities of Delonix regia Against Some Pathogenic Organisms. The primary objective was to investigate the qualitative phytochemical composition of localized Delonix regia leaf and bark specimens and quantify their corresponding in vitro inhibitory and bactericidal efficacy against common clinical isolates.The research adopted an experimental laboratory design. Fresh leaves and bark of Delonix regia were collected, authenticated, air-dried, and subjected to cold maceration using water, ethanol, and methanol as solvents. Qualitative phytochemical screening was conducted using standard diagnostic colorimetric assays. The crude extracts were then tested against a panel of pathogenic organisms—comprising Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans—using the agar well diffusion method at varying concentrations ($25\text{ mg/mL}$ to $100\text{ mg/mL}$). The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) were determined via macro-broth serial dilution methods.