COLLABORATIVE IMPACT OF CADMIUM AND LEAD ON CELLULASE ACTIVITY IN FERMENTED CASSAVA PULP EMPLOYED FOR BIOETHANOL SYNTHESIS
Abstract
The study examined the collaborative impact of cadmium (Cd) and lead (Pb) contamination of cassava pulp on cellulase activity during bioethanol production. The study made use of the Box-Behnken design, where three variables namely Cd concentration (0-100 µM), Pb concentration (0-100 µM) and fermentation time (24-72 h) were taken into consideration thereby generation seventeen experimental runs (N=17) at 30°C and pH 5. To confirm the cellulase activity, 30ml of inoculum from the activated yeast peptone dextrose (YPD) broth, 10 mL each of the contaminants, and 50 mL of hydrolysate from the pretreated cassava flour were subjected to fermentation for 24, 48 and 72 h respectively. From the study’s findings, cellulase activity was shown to be inhibited by a discernible interaction between Pb and Cd at p
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