EVALUATION OF THE COMBINED EFFECTS OF FATTY DIET AND ADRENALINE ON PROTHROMBIN (PT) AND ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT) IN ADULT FEMALE WISTAR RATS

This study was conducted to evaluate the combined effect of a fatty diet and adrenaline on Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT) in adult female Wistar rats. Alterations in coagulation parameters are critical indicators of hemostatic balance and can reflect the potential risk of thrombotic or bleeding disorders. A total of four healthy adult female Wistar rats were used for the study, divided into two groups: a control group fed with a standard laboratory diet and a test group fed with a fatty diet and administered adrenaline intraperitoneally. The experimental period lasted for 28 days. At the end of the exposure period, blood samples were collected through cardiac puncture under light anesthesia and analyzed using a semi-automated coagulation analyzer following standard laboratory procedures. The results revealed a notable decrease in both PT and aPTT values in the test group compared to the control group. Specifically, the mean PT for the control group was 23.67 seconds, while the test group recorded 17.33 seconds. Similarly, the mean aPTT was 49.33 seconds in the control group and 26.67 seconds in the test group. These findings suggest that the combination of a fatty diet and adrenaline may significantly induce a hypercoagulable state by shortening clotting times. In conclusion, the study highlights the potential interaction between high-fat consumption and stress-related hormones in accelerating coagulation, which may predispose individuals to thrombotic conditions. Further research is recommended to investigate the underlying mechanisms and long-term effects of these factors on blood coagulation. Keywords: Fatty diet, Adrenaline, Prothrombin time (PT), Activated partial thromboplastin time (aPTT), Coagulation, Wistar rats, Hypercoagulabilit