ISOLATION AND IDENTIFICATION OF PATHOGENIC BACTERIA FROM FOMITES IN IMO STATE POLYTECHNIC, OMUMA

ABSTRACT The transmission of infection through inanimate objects constitutes a major threat to public health especially in the developing countries. This study was carried out to isolate and identify potential bacteria reservoirs that may be responsible for infection in Imo Stata Polytechnic Omuma. A total of 100 swabs were taken from computer keyboards, work benches, door handle, file folders and mobile phones. Bacteria were isolated from these fomites using conventional microbiological techniques. The isolates obtained were examined and identified by colonial morphology, Gram reaction and biochemical characteristics. Antimicrobial susceptibility was performed on the isolates by paper disc diffusion method. A total of 153 bacteria were isolated. Staphylococcus aureus was the most predominant bacteria 72 (47.1 %) followed by Escherichia coli 31(20.3 %) and the least Pseudomonas aeruginosa. Ciprofloxacin was highly effective in both Gram negative and Gram-positive bacteria. The presence of these bacteria of public health importance on the sampled surfaces may probably pose a significant public health risk to the school community and its surrounding. Proper hand hygiene and disinfection of the school environment is recommended.