MICROBIAL DIVERSITY STUDY OF CRUDE OIL CONTAMINATED SOIL TREATED WITH PALM BUNCH ASH
Abstract
Crudeoilcontaminationofsoilposessignificantenvironmentalandhealthrisks.Thisstudyinvestigatestheefficacyofpalmbunchash(PPA)asalow-cost,eco-friendlyamendmentforremediatingcrudeoil-contaminatedsoil.Laboratoryexperimentswereconductedwithsoilsamplescontaminatedwith10mlofcrudeoilandamendedwith1%,5%and10%palmbunchash.ThebiodegradationprocesswasmonitoredbymeasuringmicrobialactivityandpetroleumhydrocarbondegradationonNutrientAgarandidentifyingtotalpetroleumhydrocarbondegradingmicrobesonBushnell-HaasAgar.Biochemicaltestwasemployedtoidentifykeymicrobesinvolvedincrudeoilbiodegradationinthesoil.ResultsshowthatPPAsignificantlyenhancedsoilpH,nutrientavailability,andmicrobialactivity.ThediversityindexofisolatesinNutrientagaris0.9with20%relativeabundance,andlowevenness.simillarlarly,inBushnell-Haasmineralsaltmediimedium,themicrobialdiversityindexiofisolatesis0.86,lowevenness,and25%relativelyabundant.ThemicroorganismsprimarilyinvolvedinthebiodegradationweredeterminedtobeMicrococcussp.,bacillussp.,V.parahaemolyticansspandEscherichiacoli..Thisstudydemonstratesthepotentialofpalmbunchashasasustainable,cost-effectivetoolformitigatingcrudeoilcontaminationinsoil,offeringvaluableinsightsforenvironmentalremediationandwastemanagementstrategies.
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