THE INFLUENCE OF VITAMIN E ON RENAL INFLAMMATORY ASSESSMENT OF FEMALE WISTAR RATS EXPOSED TO RESTRAINT STRESS AND ORAL CADMIUM CHLORIDE ADMINISTRATION

Environmental toxins have been identified as the primary cause of human health risks, which has an effect on the general health condition of those exposed to them. Stress is a state of mental or emotional strain resulting from the bodys response to a perceived threat or challenge. Cadmium, an heavy metal has been identified as a key contributor to illness among these toxicants and also the cause of disease affecting a variety of tissues including the renal system (kidney). Vitamin E has also been found to aid and protect cells from damages. The goal of this study is to evaluate the influence of Vitamin E on renal inflammation of female Wistar rats exposed to restraint stress and oral cadmium chloride administration. Forty-two (42) female Wistar rats were divided into 7 groups and they were assigned into control (CTL), restraint stress alone (RSS) cadmium chloride alone (CCC), restraint stress + cadmium chloride (RSC), cadmium + Vitamin E (CCV), restraint stress + cadmium + Vitamin E (RCV) and restraint stress + Vitamin E (RSV). The experimental groups were exposed to restraint stress using wire mesh and also inoculated with cadmium chloride depending on body weight, and also administered Vitamin E, orally. After 21 days of inoculation, the animals were anesthetized using chloroform and sacrificed, blood samples were collected via cardiac puncture and kidneys were for renal Interleukin-6 (IL-6), Tumor Necrosis Factor-α (TNF-α), Myeloperoxidase and Prostaglandin E2 assessment. Data was analyzed using GraphPad Prism v5.0 and the results were presented as mean ± Standard Error of Mean (SEM) and Analysis of Variance (ANOVA) was used to determine the statistical significance among the groups using p-value set at P