PREVALENCE OF CARBAPENEM RESISTANT KLEBSIELLA SPP ISOLATED FROM BEEF AND ABATTOIR ENVIRONMENT AT CENTRAL ABATTOIR AKINYELE, IBADAN.
Abstract
The emergence of carbapenem-resistant Klebsiella pneumonia (CRKP) represents a critical public health threat due to limited treatment options. Abattoirs, as intersections of animal, human, and environmental pathways, are potential reservoirs for the dissemination of such multidrug-resistant bacteria into the food chain. This study aimed to determine the prevalence and antimicrobial resistance profiles of Klebsiella species, particularly focusing on carbapenem resistance, isolated from beef and the environment at the Central Abattoir Akinyele, Ibadan. A cross-sectional study was conducted involving the collection of 60 faecal, 20 beef, and 2 water samples. Samples were processed using standard microbiological methods for the isolation and biochemical identification of K. pneumoniae. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method against 18 antibiotics, including meropenem and ertapenem, following CLSI (2020/2023) guidelines. K. pneumonia was isolated from all sample types, with the highest prevalence found in faecal samples (35%), followed by beef (20%) and water (50%). Alarmingly, all isolates (100%) were resistant to meropenem, and 75% were resistant to ertapenem, confirming the presence of CRKP. The isolates exhibited extensive multidrug resistance (MDR), showing high resistance to cephalosporins (ceftriaxone, cefepime: 100%), macrolides (erythromycin: 100%), tetracyclines (50-75%), and aminoglycosides (streptomycin: 75-100%). In contrast, fluoroquinolones (ciprofloxacin and ofloxacin) demonstrated higher susceptibility, suggesting they may currently be more effective therapeutic options. This study reveals a high prevalence of carbapenem-resistant and multidrug-resistant K. pneumonia within the Central Abattoir Akinvele. The abattoir environment, particularly faecal matter, serves as a Significant reservoir for these critical pathogens. The study demonstrates a high prevalence of carbapenem-resistant and multidrug-resistant Klebsiella pneumoniae in the abattoir, identifying faecal matter and contaminated surfaces as major reservoirs.
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