HEMOLYTIC ACTIVITY AND ANTIBIOTICS SUSCEPTIBILITY PROFILE OF BACTERIAL ISOLATES FROM NASAL SECRETION OF YELWA PRIMARY SCHOOL AND SHUARE PRIMARY SCHOOL, MUBI, ADAMAWA STATE.

Student: Hulfa Emmanuel
Supervisor: Mrs Maryam Danguma
Co-Supervisor: Dr Samuel Waja
HOD: Dr Demshemino Ph Moses
Department of Biological Science(s)
Science and Technology
Federal Polytechnic, Mubi, Adamawa State

Abstract

ABSTRACT Nasopharyngeal colonization by bacteria is a prerequisite for progression to respiratory disease and an important source of horizontal spread within communities. The study aimed to isolate and identify bacterial flora of nasal discharge among school-aged children, and their resistance to commonly used antibiotics. Nasal discharge collected using swab stick were enriched in peptone water, and subsequently introduced into blood agar, and MacConkey agar, and the bacterial isolates identified by standard protocol. Of the 100 nasal swabs analysed, 49 yield bacteria growth on blood agar, and were all confirmed to be Staphylococcus aureus. Of these, 29 (58.0%) and 20(40.0%) were from Yelwa and Shuare primary schools respectively. β-haemolysis was detected in 33(67.3%) S. aureus. 42(85.7%) of the S. aureus were resistant to at least one antibiotic, and resistance to cefuroxime (65.3%) was the highest, and least to rifampicin (8.2%). The nasal discharge of many school-aged children contains MDR S. aureus which could not only cause endogenous infection but also be a source of transmission to others.

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