ISOLATION, IDENTIFICATION, AND CHARACTERIZATION OF PATHOGENIC MICROORGANISMS ASSOCIATED WITH MOBILE PHONE

ABSTRACT Mobile phones have become indispensable tools in daily life, offering convenience in communication, learning, and work. However, their constant handling and exposure to different environments make them potential carriers of pathogenic microorganisms. This study aimed to isolate, identify, and characterize microorganisms present on the surfaces of mobile phones. Swab samples were collected from different mobile phones, including iPhone 11 Pro and iPhone XS, using sterile cotton swabs dipped in sterile distilled water. The samples were inoculated into nutrient agar using the pour plate method and incubated at 37°C for 24 hours. Colony morphology was recorded, including shape, elevation, size, surface, and pigmentation. Further identification was carried out through Gram staining, capsule staining, spore staining, and biochemical tests such as sugar fermentation (glucose, fructose, lactose, maltose, sucrose), catalase, and coagulase tests. The results revealed the presence of Gram-positive rods, some of which were spore-forming and capsule-producing. Biochemical tests showed that most isolates fermented multiple sugars, and catalase-positive reactions indicated the presence of organisms like Bacillus cereus. Morphologically, colonies ranged from circular to irregular with smooth surfaces and varied pigmentation, suggesting microbial diversity. These findings highlight the risk of microbial contamination on frequently handed personal device regular disinfection proper hygiene practices and increase public awareness are essential to reduce the potential transmission of infection agent through mobile phone particularly in health care and food handling environment.