AMELIORATIVE EFFECT OF BLACK SEED OIL ON CHEMICAL NOCICEPTION AND CATALASE IN VINCRISTIN-INDUCED PERIPHERAL NEUROPATHY IN DROPHILA MELANOGASTER (HARWICH STRAIN)
Abstract
Vincristine, a widely used chemotherapeutic agent, is associated with dose-limiting peripheral neuropathy mediated by oxidative stress and altered nociceptive pathways. This study evaluates the ameliorative potential of black seed (Nigella sativa) oil on vincristine-induced neuropathic alterations using Drosophila melanogaster (Harwich strain). Adult flies were exposed to vincristine, black seed oil, or their combination for seven days. Chemical nociception was assessed using the acetic-acid-based avoidance assay, while catalase activity was quantified as a biomarker of oxidative stress. Vincristine significantly increased nociceptive sensitivity and decreased catalase activity compared to control groups. Co-treatment with black seed oil markedly improved nociception thresholds and restored catalase levels toward baseline values. These findings suggest that black seed oil exerts neuroprotective effects through antioxidant mechanisms, offering potential therapeutic relevance in vincristine-induced peripheral neuropathy.
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