ANTIMICROBIAL PRODUCING BACILLUS SPECIES FROM WASTE DUMP SITES IN ADO EKITI
Abstract
Bacillus species are an important source of fine biochemical, antibiotics and insecticides. This study was therefore carried out to determine the antimicrobial producing bacillus species from waste dump sites in Ado-Ekiti. Soil samples were collected from 6 different dump sites in Ado-Ekiti metropolis. Total bacteria plate counts were carried out on nutrient agar using pour plate techniques. The bacterial growth were identified using colonial morphology, gram staining and biochemical characterization. The Antagonistic effect of the cell free supernatant extract (CFS) of Bacillus spp isolated were tested against clinical bacterial and fungal isolates; Escherichia coli, Staphylococcus aureus, Salmonella typhi, Candida albicans, Candida tropicalis using disc diffusion techniques. The total bacterial plate count of the six soil samples from dumpsites ranges from 2.5 x107 cfu/g to 3.4 x 107 cfu/g. Three different species of Bacillus were isolated with Bacilluscereus having the highest isolation rate of 2(50.0%), followed by Bacilluslicheniformis and Bacillusmycoides. The cell free supernatant (CFS) of Bacilluslicheniformis showed the highest antibacterial effect against clinical bacterial and fungal isolates at 50 µl and 40µl. The CFS of Bacilluscereus showed antagonistic effects on only Escherichia coli and Staphylococcus aureus. However, the need for continuous search of the new therapeutic agents from nature is necessary. Therefore, Bacilluslicheniformis with antagonistic effect on both bacterial and candida species might be a very good source of antimicrobial agent.
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