EVALUATION OF PHYTOCHEMICAL PROFILE AND ANTIMICROBIAL PROPERTIES OF CYMBOPOGON CITRATUS

Student: Matthew Ogwuche
Supervisor: Prof S. Wasagu R.
HOD: Dr Lawal Almustapha
Department of Biochemistry and Molecular Biology
Chemical and Life Sciences
Usmanu Danfodio University, Sokoto, Sokoto State

Abstract

this study investigates the phytochemica composition and antimicrobial activity of Cymbopogon citratus (lemon grass) extract to evaluate its potential as a natural therapeutic agent. The phytochemical screening revealed the presence of tannins, flavonoids, saponins, alkaloids, glycosides, volatile oils, and other bioactive compounds, with tannins (+++), flavonoids (+++), and saponin glycosides (+++) being particularly abundant. Quantitative analysis showed high concentrations of tannins (450.0 mg/g) and flavonoids (300.0 mg/g), while alkaloids were present at 13.1 mg/g- The antimicrobial activity was assessed against Staphylococcus aureus, Escherichia coli, and Aspergillus niger using the agar well diffusion method. The extract exhibited significant antibacterial activity against S. aureus (26.0 ± 0.94 mm zone of inhibition at 90 mg/ml) and moderate activity against E. coli, while no activity was observed against Aspergillus niger. These findings confirm the antibacterial potential of Cymbopogon citratus, particularly against Gram- positive bacteria, supporting its traditional use in treating microbial infections. The study concludes that Cymbopogon citratus is a promising source of bioactive compounds with significant antibacterial properties. Further research is recommended to explore its full antimicrobial

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