ELISA TEST FOR THE DETECTION OF CUCUMBER MOSAIC VIRUS IN OKRA IN AKPABUYO, CROSS RIVER STATE

Student: Faith Ojochide Monday
Supervisor: Prof Essien Archibong Okon
HOD: Prof Essien Archibong Okon
Department of Plant Biology and Biotechnology
Biological Sciences
University of Cross River State, Calabar

Abstract

ABSTRACT Okra (Abelmoschus esculentus L.) also known as lady’s finger in England is an important vegetable crop of the family Malvaceae growing throughout the tropical and warm temperate regions of Asia, Africa, and the USA for food, fibre, Gum, starch, spices, and medicinal purposes. The viral disease is a major constraint to okra production worldwide. A visit to some okra farms during the 2023 planting season in Akpabuyo, Cross River State revealed evidence of wide spread virus infection with infected crops exhibiting mosaic, mottling, and leaf malformation resulting in poor yield. Infected leaf samples were collected and tested against Cucumber Mosaic Virus (CMV), Okra Leaf Curl Virus (OkLCV), Okra Mosaic Virus (OkMV), and Black eye Cowpea Mosaic Virus (BiCMV) antiser. Samples tested positive for CMV while there was no reaction against OkMV, (OkLCV) and BiCMV. This study has revealed that CMV is widespread in Akpabuyo, Cross River State.

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