ANTIOXIDANT ENZYME ASSAY OF STREPTOZOTOCIN INDUCED FEMALE DIABETIC ALBINO RATS TREATED WITH BIOSYNTHESIZED SLIVER NANO PARTICLES
Abstract
Diabetes mellitus (DM) is a serious metabolic disorder resulting from a defect in insulin
secretion and action. Demand of silver nanoparticle (AgNPs) is increasing rapidly in many of
the streams like in medical, pharmaceutical healthcare, food, consumer, cosmetics etc. We
investigated the antioxidant enzyme assay of STZ induced female diabetic Albino Wister rats
treated with biosynthesized silver nanoparticle. Thirty female (30) albino Wistar rats
weighing (120-230g) were obtained from the University animal house and assigned to five (V)
groups with 6 rats in each group after feeding and acclimatization for two weeks with
commercially purchased pelletized animal feed. Group II-V were induced intraperitoneally
with STZ at a dose of 60mg/kg after an overnight meal fast and observed for 48hrs to induce
diabetes before treatment. Blood plasma glucose was tested with one touch glucometer using
blood collected from tail puncture. Random blood glucose of 200 mg/dL and above was
confirmation for diabetes induction. Animal groups were labelled as follows; Group I:
Normal control (NC), Group II: Diabetic control (DC), Group III: Standard control (MET),
Group IV: Silver nanoparticle (AgNO;) and Group V: Guava leaves extract (GV). At the end
of the 14-days treatment period, study animals were sacrificed after being anesthetized with
50:50 chloroform and diethyl ether. Blood was collected through cardiac puncture for
catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD). The result analysis
showed significant increase (p
Full-Text Access Notice
In accordance with the NERD Policy on promoting peer-reviewed publication, public access to the full text of a project, thesis or dissertation is restricted for three years, allowing the author and supervisors sufficient time to pursue peer-reviewed publication.
During this period, researchers with legitimate academic or research purposes may request authorisation directly from the author to enable NERD to release the indexed full texts of the work using the form below.